性能參數(shù)
分子別名(Synonyms) |
GDNFR; GFRA1;GDNFRA; RET1L |
表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source) |
Human GFR alpha 1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp25-Ser424.[Accession | NP_665736.1] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result. |
內(nèi)毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
純度(Purity) |
> 95% as determined by SDS-PAGE and HPLC. |
活性(Activity) |
ELISA Data: Immobilized Human GDNF, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 5.0ng/ml determined by ELISA. |
制劑(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重構(gòu)方法(Reconstitution) |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
儲(chǔ)存條件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項(xiàng)
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
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